Myrosinase is an enzyme that activates a group of metabolites, namely glucosinolates, which are involved in the defense against herbivores and pathogens. In Arabidopsis thaliana, THIOGLUCOSIDE GLUCOHYDROLASE 1 (TGG1) and TGG2 are two myrosinases that accumulate in specialized myrosin cells of rosette leaves. Here, we show that prolonged exposure to the wounding hormone methyl jasmonate (MeJA) enhances TGG1 and TGG2 expression independent of the canonical jasmonic acid (JA) signaling pathway. We found that airborne MeJA treatment for up to 5-days enhanced both TGG1 and TGG2 gene expression and their protein levels in Arabidopsis leaves. TGG1 and TGG2 gene expression did not stop in two JA signaling pathway mutants, namely coi1-16 and myc2,3,4, after 5-days of MeJA treatment, although other typical JA responses were completely stopped in these two mutants. FAMA is a transcription factor that participates in the specification of myrosin cell development, but it does not appear to be involved in TGG1 and TGG2 gene expression regulation in the 5-day MeJA treatment, as its expression did not increase. Taken together, our results suggest the existence of an alternative JA signaling pathway that is activated by long-term exposure to MeJA.