Demography analyses
DNA sequence polymorphism (DnaSP) v6.12.03 was used to gather baseline statistics of gene region for each species with more than four representatives. Ambiguous nucleotides were removed, and all other settings left on default. The basic statistics recorded were as follows: nucleotide diversity (π), gene diversity (Hd), number of haplotypes (H), mean number of pairwise differences (k) and number of segregating sites (S). Tajima’s test and Fu and Li’s tests were also run . Tajima’s D compares the number of segregating sites (S) and the mean pairwise differences between different sequences . Fu and Li’s D* is similar but compares the total amount of nucleotide variations to the number of singleton mutations, this was done without an outgroup . Both tests are statistical tests of neutrality and are used to determine historic population demographic changes .