Figure 4. Polyadenylation signals detected by direct RNA sequencing 
A. Volcano plot depicting log2 Fold Change (log2FC) for genes with significant poly (A) tail. The x-axis displays the log2FC values for each gene, while the y-axis shows the negative log-adjusted p-value (p-adjusted). The horizontal dashed line represents the negative logarithmic p-adjusted cutoff value (0.05), and the vertical line equals the value of 0 log2FC. Red points indicate statistically significant genes, while gray points represent non-significant genes.
B. Boxplot comparing the distribution of poly(A) tail lengths in the CL.22730.2 transcript across study groups, water (blue) and land (orange). The boxplot depicts the median, first and third quartiles (lower and upper hinges), largest and smallest value (upper and lower whisker).
C. Poly(A) tail length profiling of Riccia fluitans mRNA depending on the living environment. A density distribution plot is shown for the mRNA of all transcripts detected in Riccia fluitanscells in the aquatic environment (blue) and in the terrestrial environment (orange). The vertical dashed lines represent the median poly(A) tail length (in nucleotides).
D. Scatter plot of poly(A) tail length for differentRiccia fluitans clones, representing all transcripts from poly(A) tail length profiling. Each point represents the length of poly(A) from a clone grown in either an aquatic (blue) or terrestrial (land) environment.