Fig. 6. DACA maintains mitochondrial homeostasis by regulating mitochondrial autophagy. (A-B) Representative micrographs of JC-1 staining in SH-SY5Y cells. Scale bar 100 mm. (B). The fluorescence analysis of JC-1 in SH-SY5Y cells on a fluorescence plate reader at Ex/Em = 514/529 nm and Ex/Em = 585/590 nm(B) (n = 5 per group). (C-D) Immunofluorescence analysis of mitochondrial superoxide in SH-SY5Y cells. Scale bar 100 mm. Data are shown as representative pictures(C) and mean fluorescence intensity(D) (n = 5 per group). (E-H) Immunoblot analysis of p62, PINK1, LC3, Bax and Bcl-2 in SH-SY5Y cells and primary neurons. Data are shown as representative pictures (E, G) and quantified relative expression (F, H) (n = 5 per group). Data shown are mean ± SEM (n = 5); *p < 0.05, **p < 0.01 vs. control;#p < 0.05, ##p < 0.01 vs. MPP+. NS. not significant.