Figure 4. Effect of AdipoAI treatment on adiponectin subunits
expression and hepatic microarchitecture in liver tissues. (A, B & C)
mRNA Gene expression profiles of Ppargc1a, AdipoR2 in liver of 21 weeks
old DIO mice treated with CMC (0.5%), AdipoAI (25 mg/kg) and AdipoRON
(50 mg/kg) administered biweekly respectively (D) Western blot analysis
phosphorylation of AMPK in liver samples treated with indicated amount
of AdipoAI and AdipoRON (E) Immunohistochemistry staining profiles of
p-AMPK levels in liver tissues from mice study models showing increase
AMPK localization in AdipoAI treated groups (E, F and G) mRNA expression
of inflammatory cytokines IL-6, TNF-a, IL-1B in liver tissues treated
with CMC, AdipoAI, AdipoRON biweekly for four weeks (H) Hematoxylin and
eosin staining depict improved hepatic details compared to control. Data
were analyzed by using ordinary 1-way followed by multiple comparison
between groups. all the values are presented as mean ± SD with three
independent experiments sets with *p<0.05; **p<0.01,
***p<0.001.
.