CCK8 assay
CCK-8 assay was performed as described previously (Qiu et al., 2021) briefly C2C12 cells (0.05X104 cells/well) were plated in 96 well plate overnight (day 0), following AdipoAI and AdipoRON treatment for 24hours. After 24hours treatment CCK-8 (cell counting kit) (Dojindo, Rockville, MD, USA) assay was performed according to manufacture instruction the media was discarded carefully and 10ul of CCK-8 was added to the plates and incubated for 2h in incubator. The absorbance was recorded at 450nmn and calculated with respect to nontreated control groups.