CCK8 assay
CCK-8 assay was performed as described previously (Qiu et al., 2021)
briefly C2C12 cells (0.05X104 cells/well) were plated
in 96 well plate overnight (day 0), following AdipoAI and AdipoRON
treatment for 24hours. After 24hours treatment CCK-8 (cell counting kit)
(Dojindo, Rockville, MD, USA) assay was performed according to
manufacture instruction the media was discarded carefully and 10ul of
CCK-8 was added to the plates and incubated for 2h in incubator. The
absorbance was recorded at 450nmn and calculated with respect to
nontreated control groups.