Oral glucose and Insulin tolerance test
DIO Mice were starved overnight (16h), followed by oral administration of glucose (1mg/kg) were given to each group (n=6). Blood glucose levels were evaluated prior to dosing, and carefully monitored every 15 minutes time interval for a period of 120 mins, following oral administration. Insulin tolerance test (0.75 IU insulin per kg body weight) was performed in treated DIO mice models after 6 hours of fasting. Blood glucose was monitored at specific time interval (0 -120 mins) by using a sensitive glucometer.