AdipoAI increases AdipoR1-PGC1-a expression in adipose tissues.
As PGC1a (pPARGC1a) regulation is involved in lipid metabolism in muscle adipocytes (Bogacka, Xie, Bray, & Smith, 2005; Liang & Ward, 2006; Lin et al., 2002; Patti et al., 2003). We assume that AdipoAI and AdipoRON oral administration regulates adiponectin and PGC1a expression levels in white adipose tissue (WAT). In WAT AdipoAI and AdipoRON both increase AdipoR1, pPARGC1a, PPARA gene expression levels in comparison to DIO mice (Fig 5A, C & D). Higher gene expression of all the three tested gene was observed in AdipoAI treated group, suggesting that AdipoAI could activate adiponectin signaling pathways through AdipoR1 and lower doses of AdipoAI may influence the lipid metabolism in WAT which could be a potential target.