Animal experiment
All the animals reported were approved by the animal care committee of
Tufts University, Boston, MA. Diet induced obese (DIO, C57BL/6) male
mice at 17 weeks of age were purchased from Jackson’s laboratory and
housed control pathogen free conditions (22°C) 12h light-dark cycle. One
week after arrival mice were divided into three groups (n=6 per group)
as follows CMC-Na (Carvoxymethylcellulose-sodium salt, thermoscientific,
A18105.36) 0.5% (HFD group), AdipoAI 25mg/kg (HFD along with AdipoAI),
AdipoRON 50 mg/kg (HFD along with AdipoRON) were fed with high fed diet
consisting of 58% fat, 25.6% carbohydrates and 16.4% protein with
access to water for 4 weeks. Small molecule AdipoAI (25 mg/kg) and
AdipoRON (50 mg/kg) were dissolved and administered in 0.5% CMC-Na
(carboxymethyl cellulose) orally by 18G curved gavage as reported by
others (Shi et al., 2013).Oral treatment was introduced at 18 weeks and
continued for 4 weeks (2X/week) and mice weights were recorded carefully
every week as illustrated in concept map Fig 3A. At the end of
3rd and 4th week oral glucose and
insulin tolerance test were performed.