Figure 4. Effect of AdipoAI treatment on adiponectin subunits expression and hepatic microarchitecture in liver tissues. (A, B & C) mRNA Gene expression profiles of Ppargc1a, AdipoR2 in liver of 21 weeks old DIO mice treated with CMC (0.5%), AdipoAI (25 mg/kg) and AdipoRON (50 mg/kg) administered biweekly respectively (D) Western blot analysis phosphorylation of AMPK in liver samples treated with indicated amount of AdipoAI and AdipoRON (E) Immunohistochemistry staining profiles of p-AMPK levels in liver tissues from mice study models showing increase AMPK localization in AdipoAI treated groups (E, F and G) mRNA expression of inflammatory cytokines IL-6, TNF-a, IL-1B in liver tissues treated with CMC, AdipoAI, AdipoRON biweekly for four weeks (H) Hematoxylin and eosin staining depict improved hepatic details compared to control. Data were analyzed by using ordinary 1-way followed by multiple comparison between groups. all the values are presented as mean ± SD with three independent experiments sets with *p<0.05; **p<0.01, ***p<0.001.
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