Animal experiment
All the animals reported were approved by the animal care committee of Tufts University, Boston, MA. Diet induced obese (DIO, C57BL/6) male mice at 17 weeks of age were purchased from Jackson’s laboratory and housed control pathogen free conditions (22°C) 12h light-dark cycle. One week after arrival mice were divided into three groups (n=6 per group) as follows CMC-Na (Carvoxymethylcellulose-sodium salt, thermoscientific, A18105.36) 0.5% (HFD group), AdipoAI 25mg/kg (HFD along with AdipoAI), AdipoRON 50 mg/kg (HFD along with AdipoRON) were fed with high fed diet consisting of 58% fat, 25.6% carbohydrates and 16.4% protein with access to water for 4 weeks. Small molecule AdipoAI (25 mg/kg) and AdipoRON (50 mg/kg) were dissolved and administered in 0.5% CMC-Na (carboxymethyl cellulose) orally by 18G curved gavage as reported by others (Shi et al., 2013).Oral treatment was introduced at 18 weeks and continued for 4 weeks (2X/week) and mice weights were recorded carefully every week as illustrated in concept map Fig 3A. At the end of 3rd and 4th week oral glucose and insulin tolerance test were performed.