Figures
Figure 1: Comparative in vitro studies of AdipoAI and AdipoRON to determine cellular toxicity and mRNA gene expression levels: (A) Cellular toxicity and behavioral patterns of C2C12 myotubes treated with AdipoAI and AdipoRON (0-10 mM) after 24 hrs incubation by using CCK8 assay; determined at 450 nm absorbance spectra. Cell cytotoxicity test reveals IC50 values of AdipoAI and AdipoRON. Quantitative gene expression analysis of (B) AdipoR1 (C) AdipoR2 (D) Ppargc1a in C2C12 myotubes treated with AdipoAI and AdipoRON (0-5 uM) after 1.5 hours of treatment. (E) Adiponectin signaling in differentiated C2C12 myotubes treated with different concentrations of AdipoAI and AdipoRON for 1.5 hours with respect to controls (untreated). . Statistical significance was calculated based on ordinary one-way analysis of variance (ANOVA) with multiple comparison using GraphPad Prism 9.4.1 software and all the values are presented as mean ± SD with three independent experiments sets with *p<0.05; **p<0.001.Figure 2. Oral Glucose tolerance (OGTT) and Insulin tolerance test (ITT) test at 20 and 21 weeks in diet induced obesity (DIO) mice models for accessing AdipoAI efficacy. (A) Oral glucose tolerance test tested at 20 weeks in DIO mice treated with CMC (0.5%), AdipoAI (25 mg/kg) and AdipoRON (50 mg/kg). (B) Insulin tolerance test tested at 21 weeks in DIO mice treated with CMC (0.5%), AdipoAI (25 mg/kg) and AdipoRON (50 mg/kg). Data are represented as mean ± SD of 6 mice per group.; *p<0.05, **p<0.001 compared to CMC vehicle.