Oral glucose and Insulin tolerance test
DIO Mice were starved overnight (16h), followed by oral administration
of glucose (1mg/kg) were given to each group (n=6). Blood glucose levels
were evaluated prior to dosing, and carefully monitored every 15 minutes
time interval for a period of 120 mins, following oral administration.
Insulin tolerance test (0.75 IU insulin per kg body weight) was
performed in treated DIO mice models after 6 hours of fasting. Blood
glucose was monitored at specific time interval (0 -120 mins) by using a
sensitive glucometer.