2.6 TEM
The target cells were amplified in 5ml YPDA at 30 ℃ till about
108 cells of every group. Then we centrifuged to get
the precipitations and incubated them in the DOPA medium at 30 ℃ for 3
days. Next, we centrifuged the cultures and fixed the cells with High
Efficient Pre-fixed Electron Microscope Fixative Solution (Servicebio,
Hubei, China) at room temperature for 2 h and 4 ℃ for transportation.
The subsequent experimental procedure and image-capture of TEM were
conducted by Servicebio Technology CO., LTD.