2.7 Laccase activity
We incubated the albino mutant, LAC1-knockdown and WT strains in 5ml YPDA at 30 ℃ overnight, washed the cells three times with PBS, and then transferred them to DOPA broth shaking for 3 days and 5 days. We quantified the laccase activity by the appearance of pigment in the culture supernatant with absorbance at 475nm (Thermo ScientificMultiskan GO ).
Results