2.6 TEM
The target cells were amplified in 5ml YPDA at 30 ℃ till about 108 cells of every group. Then we centrifuged to get the precipitations and incubated them in the DOPA medium at 30 ℃ for 3 days. Next, we centrifuged the cultures and fixed the cells with High Efficient Pre-fixed Electron Microscope Fixative Solution (Servicebio, Hubei, China) at room temperature for 2 h and 4 ℃ for transportation. The subsequent experimental procedure and image-capture of TEM were conducted by Servicebio Technology CO., LTD.