2.2 Construction of single-stranded DNA vectors containing
site-specific AFB1-FapyGua
To evaluate the mutagenic potential of AFB1-FapyGua in
different sequence contexts, we utilized the pSBL vector system, with
the AFB1-FapyGua-containing 11-mer oligodeoxynucleotides
inserted between the Eco RV sites in a stem region of
single-stranded pSBL, using the same conditions as previously described
(Minko et al. 2017). Vectors were purified using Amicon Ultra 100K
centrifugal filters (Merck Millipore, LTD) with TE buffer (10 mM
Tris-HCl (pH 7.4) and 1 mM EDTA).